Name: Hofman's Violet
Rating: 5 (10 reviews)

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sdf 文件

结构编辑

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HIT ID:HIT212716346
Cas No:8004-86-2
常用名:Hofman's Violet
IUPAC:N-ethyl-4-{[4-(ethylamino)phenyl][(1Z,4Z)-4-(ethylimino)cyclohexa-2,5-dien-1-ylidene]methyl}-2-methylaniline hydrochloride
IUPAC:N-ethyl-4-{[4-(ethylamino)phenyl][(1Z,4Z)-4-(ethylimino)cyclohexa-2,5-dien-1-ylidene]methyl}-2-methylaniline hydrochloride
InChI key:OKNJKIKBMQYONP-SNDLIFLWSA-N

分子式:C26H32ClN3
分子量:422.01
氢键供体数 (HBD):2
氢键受体数 (HBA):3
LogP:5.2252913
SMILES:Cl.CCNc1ccc(cc1)C(=C1C=CC(C=C1)=NCC)c1ccc(NCC)c(C)c1 |c:12,15,(;8.18,8.63,;8.18,7.09,;9.51,6.32,;9.51,4.78,;10.85,4.01,;10.85,2.47,;9.51,1.7,;8.18,2.47,;8.18,4.01,;9.51,.16,;8.18,-.61,;8.18,-2.15,;6.85,-2.92,;5.51,-2.15,;5.51,-.61,;6.85,.16,;4.18,-2.92,;4.18,-4.46,;5.51,-5.23,;10.85,-.61,;12.18,.16,;13.52,-.61,;13.52,-2.15,;14.85,-2.92,;16.18,-2.15,;17.52,-2.92,;12.18,-2.92,;12.18,-4.46,;10.85,-2.15,)|
SMILES:Cl.CCNc1ccc(cc1)C(=C1C=CC(C=C1)=NCC)c1ccc(NCC)c(C)c1 |c:12,15,(;8.18,8.63,;8.18,7.09,;9.51,6.32,;9.51,4.78,;10.85,4.01,;10.85,2.47,;9.51,1.7,;8.18,2.47,;8.18,4.01,;9.51,.16,;8.18,-.61,;8.18,-2.15,;6.85,-2.92,;5.51,-2.15,;5.51,-.61,;6.85,.16,;4.18,-2.92,;4.18,-4.46,;5.51,-5.23,;10.85,-.61,;12.18,.16,;13.52,-.61,;13.52,-2.15,;14.85,-2.92,;16.18,-2.15,;17.52,-2.92,;12.18,-2.92,;12.18,-4.46,;10.85,-2.15,)|

Hoffman's violet is specific stains for animal chromosomes. Iodinated alcohol after staining can be substituted with various acids, both organic as well as inorganic, all of which act as trapping agent preventing leaching of the dye that binds with the chromosomal DNA. RNA is not involved by this process of staining, since treatment of stained sections with cold phosphoric acid at 5 degrees C for 20--25 min and then stained also reveals perfect colouration of the chromosomes without any cytoplasmic staining.

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